Assessment of the specificity of each qPCR/RT-qPCR assay using reference viral and bacterial DNA and RNA.
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posted on 2024-03-22, 17:23 authored by Côme J. Thieulent, Mariano Carossino, Laura Peak, Wendy Wolfson, Udeni B. R. BalasuriyaEach column corresponds to one specific qPCR/RT-qPCR assay and each row corresponds to one specific reference strain of virus or bacteria. Specificity was assessed for each assay in singleplex and in multiplex formats. White cases correspond to the absence of detection while black cases correspond to DNA/RNA amplification in both singleplex and multiplex assays.
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