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Assessment of the Reproducibility of Random Hexapeptide Peptide Library-Based Protein Normalization

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posted on 05.02.2010 by Ravi C. Dwivedi, Oleg V. Krokhin, John P. Cortens, John A. Wilkins
The wide dynamic range of proteins in biological samples poses a challenge for the detection of low-abundance proteins. Recently, combinatorial hexapeptide peptide libraries have been suggested as an approach to normalization of proteins in such mixtures. We examined the reproducibility of a commercial hexapeptide ligand library for quantitative and comparative serum proteomic analysis. We also compared this technology with IgY-based affinity depletion.