Assessment of cytokine activation (mRNA expression) after high-intensity focused ultrasound (HIFU) irradiation at the silicone injection site.
The bar graph depicts the difference in cytokine activation between the control and experimental groups, as revealed by reverse transcription polymerase chain reaction (RT-PCR). The relative expression of heat shock protein (HSP)27, HSP60, HSP70, interleukin (IL)-8, toll-like receptor (TLR)2, and TLR4 were significantly increased in the experimental group that underwent HIFU treatment. The expression of transforming growth factor (TGF)-β1 was increased in the experimental group, which confirmed the trend. However, the difference was not statistically significant. (*P < 0.05, **P < 0.005, ***P < 0.0005 vs the control group).