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Apoptotic defects in LC cells.

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posted on 24.01.2020, 18:27 authored by Li-Tao Yang, Fei Ma, Hao-Tao Zeng, Miao Zhao, Xian-Hai Zeng, Zhi-Qiang Liu, Ping-Chang Yang

The lung cancer (LC) tissues were collected from LC patients (n = 18). LC cells and NC cells (marginal normal tissues; proved by a pathologist) were isolated from the tissues and exposed to cisplatin or saline (vehicle, used as a control) in the culture for 48 h to induce apoptosis. The cells were analyzed by flow cytometry, RT-qPCR and Western blotting. A, gated cells are apoptotic cells. B, summarized data of apoptotic cells in panel A. C, FasL mRNA levels. D, FasL protein levels. E-F, positive correlation between FasL mRNA and apoptotic cells after exposing to cisplatin. Data of bars are presented as mean ± SEM. Each dot in bars presents data obtained from one sample. Statistics: t test.