pone.0265327.g003.tif (2.78 MB)

Apoptotic cell death in the optic fissure is not aberrantly upregulated in the dzip1 mutant.

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posted on 2022-03-14, 17:31 authored by Sri Pratima Nandamuri, Sarah Lusk, Kristen M. Kwan

(A-F) Whole mount immunofluorescence in wild type (A-C) and dzip1ts294e mutants (D-F) for cell death (green; activated Caspase-3) and nuclei (magenta; TO-PRO-3) at 24 hpf (A, D), 36 hpf (B, E), and 48 hpf (C, F). All images are lateral views of 3-dimensional renderings. White dashed lines mark the boundary of the eye; yellow dashed lines mark the lens. Examples of cells labeled with activated caspase-3 elsewhere within the eye are indicated (white arrowheads). (G-I) Quantification of total number of activated caspase-3-positive cells within the optic fissure in the eye at 24 hpf, 36 hpf, and 48 hpf. n (embryos) for each genotype shown at the base of the graph. P-values were calculated using Welch’s t-test (M-O). Scale bar: 50 μm. D, dorsal; V, ventral; N, nasal; T, temporal.