Apoptosis of PDX-derived CRC cell lines treatment combinations.
(A) Western blot analysis of PDX-derived CRC cell lines treated with 10μM FND-4b, 5μM PI-103, and 100nM SN-38, alone and in combination versus untreated control (media alone) for 24h duration. PARP cleavage is indicated by an arrow; β-actin was used as a loading control. The images are representative of three independent experiments. In all CRC cell lines, PARP cleavage was increased as a result of FND-4b treatment compared to untreated control. (B) DNA fragmentation measured by Cell Death ELISA Assays of PDX-derived CRC cell lines treated with 10μM FND-4b, 5μM PI-103, and 100nM SN-38, alone and in combination for 48h duration. Graphic representations are the mean ± SD of DNA fragmentation plotted as absorbance (Au); each measurement was performed with 3 replicates. *p<0.05 vs. control and #p<0.01 vs. FND-4b alone.