ApWJL and LpNC8 auxotrophies detected in liquid fly HD.
(A) Heat map representing the mean ODMax reached by ApWJL or LpNC8 after 72 h of culture. Each line shows growth in a different version of the liquid HD: complete HD (first line) or HD lacking nutrient X (ΔX, lines below). Cultures were made in 96-well plates under agitation. Asterisks (*) pinpoint contradictions with our metabolic pathway automated annotations, which are explained in panel B. (B) Growth of LpNC8 in 4 versions of liquid HD: complete HD, HDΔThr, HDΔAla, and HDΔAsp in static conditions. Plot shows means with standard error based on 3 replicates by assay. Each dot represents an independent replicate. The dashed line represents the level of inoculation at t = 0 h (104 CFUs per mL). ApWJL, A. pomorumWJL; CFU, colony-forming unit; EAAFly, fly essential amino acid; HD, Holidic Diet; LpNC8, L. plantarumNC8; NALs, nucleic acids and lipids; NEAAFLY, fly nonessential amino acid; ODMax, maximal optical density.