Anti-tumor effects of X-PACT and its individual components on 4T1-HER2 cells.
A: cell viability after X-PACT (10μM 8-MOP equivalent dilution of UVADEX, 50μg/mL phosphor, 1Gy of 80kVp radiation) as determined by Guava flow cytometry. N is the number of independent measurements (different days), and error bars indicate one standard deviation. Radiation had a significantly different effect on cell viability when administered with X-PACT than with phosphor or UVADEX alone (p<0.0001 for interaction in two-way ANOVA). With X-PACT, radiation resulted in a significant decrease in cell viability relative to no x-ray dose. B: the Annexin V (+) fraction of viable cells shown in 3A. Radiation had a significantly different effect on Annexin V when administered with X-PACT, as opposed to individual components (p<0.0001). C and D: Cell viability illustrated by methyl blue staining for identical plates each receiving 1Gy of 80kVp x-rays. Each plate contained wells including no additives (control), three concentrations of phosphor only (25, 50, & 100μg/mL with DLC), UVADEX only (10uM 8-MOP equivalent dilution), and three combination X-PACT regimes.