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Anti-tumor effects of X-PACT and its individual components on 4T1-HER2 cells.

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posted on 01.09.2016, 04:55 by Mark Oldham, Paul Yoon, Zak Fathi, Wayne F. Beyer, Justus Adamson, Leihua Liu, David Alcorta, Wenle Xia, Takuya Osada, Congxiao Liu, Xiao Y. Yang, Rebecca D. Dodd, James E. Herndon II, Boyu Meng, David G. Kirsch, H. Kim Lyerly, Mark W. Dewhirst, Peter Fecci, Harold Walder, Neil L. Spector

A: cell viability after X-PACT (10μM 8-MOP equivalent dilution of UVADEX, 50μg/mL phosphor, 1Gy of 80kVp radiation) as determined by Guava flow cytometry. N is the number of independent measurements (different days), and error bars indicate one standard deviation. Radiation had a significantly different effect on cell viability when administered with X-PACT than with phosphor or UVADEX alone (p<0.0001 for interaction in two-way ANOVA). With X-PACT, radiation resulted in a significant decrease in cell viability relative to no x-ray dose. B: the Annexin V (+) fraction of viable cells shown in 3A. Radiation had a significantly different effect on Annexin V when administered with X-PACT, as opposed to individual components (p<0.0001). C and D: Cell viability illustrated by methyl blue staining for identical plates each receiving 1Gy of 80kVp x-rays. Each plate contained wells including no additives (control), three concentrations of phosphor only (25, 50, & 100μg/mL with DLC), UVADEX only (10uM 8-MOP equivalent dilution), and three combination X-PACT regimes.

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