Angiotensin—(1–7) and miRNA-149-3p repress A549 cell invasion.
Transwell chamber invasion assays of A549 cells either treated or left untreated with the heptapeptide, or transfected with miRNA-149-3p mimics or inhibitor were performed and representative fields of invaded and stained cells are shown (left). The graph presents values of the average from three independent experiments, and the error bars represent the standard deviation of the mean. ANOVA testing showed significant differences between the ability of A549 untreated culture (control) to invade the chambers and the cellular groups treated with ang-(1–7) or transfected with miR-149-3p mimics. The significance level was set at p<0.05 (***).