Anatomy of periosteal tissue prior to and following in vitro culture.

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posted on 2022-12-30, 18:48 authored by Mary M. Walker, Molly E. Baumann, John H. Alexander, Britani N. Blackstone, Christopher B. Morgan, Thomas J. Scharschmidt, Heather M. Powell

H&E stained sections of fresh porcine periosteal tissue and periosteum after 10 days in culture. Scale bar = 100 μm.

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histologic examination revealed explore culture methodology div >< p current project explores provide increased area facilitate graft incorporation total tissue size new tissue mineralization strain increased area viability via mtt mechanical strain induces human cancellous allografts floating samples serving ex vivo autogenous bone grafts mechanical expanded periosteum periosteal groups outperformed observed among groups fresh periosteum group fresh periosteum demonstrating per day strained vivo per day mechanical strain tissue area values among poor incorporation periosteal allograft daily strain porcine periosteum tissue anatomy significant increase patient ’osteogenic properties osteogenic potential natural limitation mechanical expansion ki67 staining implanted subcutaneously histological assessment highest levels freshly harvested common sequalae bone interface athymic mice alkaline phosphatase

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Anatomy of periosteal tissue prior to and following in vitro culture.

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