Analytical size exclusion and UV-vis spectrum of purified native DPP3.
(A) Analytical Size Exclusion Chromatogram of native hDPP3. DPP3 purified via IAP and IEX was applied on a Shodex silica gel-size exclusion column with a flowrate of 0.5 mL/min. Fractions belonging to peaks 1 and 2 were collected and analyzed via SDS-PAGE (inlay). (B) UV-vis spectrum of purified native DPP3 in the range of 250-550nm. The spectrum was recorded in storage buffer at room temperature using DPP3 in a concentration of 324 μg/mL.