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Analysis of membrane permeability, cell morphology and antigen expression in TransFix<sup>®</sup> treated cells.

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posted on 2016-09-07, 17:35 authored by Cecilia González, Rosa Esteban, Carme Canals, Eduardo Muñiz-Díaz, Núria Nogués
<p><b>A.</b> Staining with 7-AAD reagent at different times after treatment and comparison with equivalent fresh cells. <b>B.</b> Forward (FSC) and side scatter (SSC) parameters were acquired at several time points (shown for 120 days), and compared with fresh cells. <b>C.</b> Antigen expression levels of MELDia cells (HIRO.71 staining) or CHOLua cells (BRIC221 staining) at 120 days of treatment (fresh cells are overlaid); negative controls were MELwt or CHOwt cells stained in matching conditions.</p>

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    transfected cells antigenic propertieslow-incidence blood group antigensblood group antigensmembrane permeabilitycell linescell preservation methodsantigen expression levelsLow-Incidence Blood Group AntigensNovel Methods Facilitatinglow-incidence blood group phenotypescell stabilization methodsRBCcell culture facilities

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