Analysis of hepatic lipid homeostasis in response to MCD in culture and in vivo.
(A) Hepatic lipid accumulation in primary hepatocytes from chow-fed WT and CD18 mut mice, cultured in methionine-choline-sufficient (MCS) or methionine-choline-deficient (MCD) medium for 48 h. Values represent mean ± SE for n = 3. (B, C, D) Hepatic expression of genes pertinent to lipogenesis (B), fatty acid oxidation (C) and lipolysis (D) in WT and CD18 mut mice after 3 wk of MCD feeding in vivo, normalized to WT mice on chow diets. ACOX1, acyl-CoA oxidase-1; ATGL, adipose triglyceride lipase; CPT1, carnitine palmitoyl transferase-1; DGAT2, diacylglycerol transferase-2; FAS, fatty acid synthase; PNPLA3, patatin-like phospholipase domain containing 3; PPARa, peroxisome proliferator activated receptor-α; SCD1, stearoyl-CoA desaturase-1; VLCAD, very long chain acyl dehydrogenase. Values represent mean ± SE for n = 8–14. * P < 0.05 vs. WT.