Analysis of SPP interaction of a double fluorescence-tagged HO-1 in HEK293 cells.
(A) Confocal laser scanning analysis of HO variants under normoxic or hypoxic conditions (1% O2, 48 h) and in co-transfection with wild type HA-tagged SPP or the inactive mutant. Figure shows representative data from five pictures per sample out of three independent experiments. Bar represents 20 μm. (B) Co-immunoprecipitations with HA-antibody, detected with GFP-antibody. Western blots of cell extracts used for co-immunoprecipitation were incubated with GFP-antibody to detect HO variants and were incubated with HA-antibody to detect SPP as homodimer at around 95 kDa. Representative blots of one out of three independent experiments are shown. WT: wild type, mut: inactive mutant, right: ladder [kDa].