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Analysis of PrPSc in the CNS of CWD infected Tg and Gt mice by histoblotting and immunohistochemistry.

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posted on 26.07.2021, 18:00 authored by Jifeng Bian, Sehun Kim, Sarah J. Kane, Jenna Crowell, Julianna L. Sun, Jeffrey Christiansen, Eri Saijo, Julie A. Moreno, James DiLisio, Emily Burnett, Sandra Pritzkow, Damian Gorski, Claudio Soto, Terry J. Kreeger, Aru Balachandran, Gordon Mitchell, Michael W. Miller, Romolo Nonno, Turid Vikøren, Jørn Våge, Knut Madslien, Linh Tran, Tram Thu Vuong, Sylvie L. Benestad, Glenn C. Telling

Histoblotted coronal brain sections at the level of the hippocampus and thalamus (A., C., E., G.,) and immunohistochemical (IHC) analysis (B., D., E., F., H.,) of PrPSc deposition in the region of the hippocampus of Q226 mice infected with North American moose CWD (A., B.); Norwegian moose isolate M-NO2 (C., D.); Norwegian reindeer (E., F.); and adapted Norwegian moose CWD, M-NO2Ad (G., H.). Arrow in I. indicates location of M-NO2Ad PrPSc plaque aggregates in the cortex at the level of the hippocampus during second passage of M-NO2 in TgQ226 mice. J., section from the region of the hippocampus of uninfected TgQ226 mice. PK treated histoblots were probed with mAb PRC5. IHC sections were probed with Fab D18. Bar in B., D., F., H., J. = 10 μM; bar in I. = 200 μM.

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