Analyses of FKH1 motifs at FHA-SORT-positive Cen-associated origins.

The analyses in Fig 4, main text, were applied to Cen-associated origins that were FHA positive. (A) The fraction (y-axis) of origins within the indicated group (x-axis) containing at least one match to the indicated motif within the indicated origin regions for the relevant FHA-regulated origin groups classified by SortSeq. “5’ FKH1-T” queried nucleotides -150 through -11 for FKH1-T motif matches, while “3’ FKH1-A” queried nucleotides +41 through +150 for FKH1-A matches. The “ORC-overlapping” region encompassed nucleotides -10 through +40 and was queried for FKH1-T motifs. The horizontal line indicates the fraction of all confirmed origins (n = 393) that contained a match to the queried FKH motif in the origin region under assessment. The enrichment or depletion of a given motif in any given origin group was challenged against the fraction of that motif in all confirmed origins using the hypergeometric distribution function. Significant P-values are denoted by asterisks (*, P < 0.05; **, P < 0.001; ***, P < 0.0001). In these analyses, 150 bp regions 5’ and 3’ of the ORC site were queried. (B) The cumulative fraction of origins (y-axis) in the indicated groups containing a 5’FKH-T (left) or a 3’FKH-A (right) after traversing the indicated number of nucleotides from the ORC site (x-axis). Nucleotide positions that reached P-value significance values of 0.01 are indicated by gray cross marks derived from hypergeometric distributions where at each position, the fraction of origins in the queried collection that contained a match by that nucleotide position was reached is compared to the fraction of all confirmed origins (n = 393) that contained a match by the same position. (C) Summary of the 50% accumulation point for the analyses in (B).

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