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Amino acid analysis of S. aureus and B. anthracis and PGN preparations before and after proteinase K digestion.

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posted on 23.02.2018 by Marybeth Langer, Alanson W. Girton, Narcis I. Popescu, Tarea Burgett, Jordan P. Metcalf, K. Mark Coggeshall

S. aureus amino acids were analyzed before (A) and after (B) Proteinase K digestion. The final S. aureus PGN product was additionally treated with HF, extracted with SDS, and analyzed (C). B. anthracis amino acids were analyzed before (D) and after (E) proteinase K digestion. Two separate PGN preparations for each species were analyzed, with three replicates of each sample. Data are expressed as the mean ± SEM of amino acid content, normalized to lysine for S. aureus PGN and to DAP for B. anthracis PGN.

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