Amino acid analysis of S. aureus and B. anthracis and PGN preparations before and after proteinase K digestion.
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S. aureus amino acids were analyzed before (A) and after (B) Proteinase K digestion. The final S. aureus PGN product was additionally treated with HF, extracted with SDS, and analyzed (C). B. anthracis amino acids were analyzed before (D) and after (E) proteinase K digestion. Two separate PGN preparations for each species were analyzed, with three replicates of each sample. Data are expressed as the mean ± SEM of amino acid content, normalized to lysine for S. aureus PGN and to DAP for B. anthracis PGN.