AmiA and AmiC contribute to S. Tm gut colonization.
Streptomycin-treated C57BL/6 mice (n = 8 or 10) were infected for 8 days with 1:1 mixture (total 5×107 CFU intragastrically) of S. Tm ssaV and ssaV tatC mutants, or ssaV and ssaV amiA mutants, or ssaV and ssaV amiC mutants, or ssaV and ssaV amiA amiC mutants. (A, D, G and J) CI of S. Tm loads in feces at 1, 3, 6, and 8 dpi. (B, E, H and K) CI of S. Tm loads in cecum at 8 dpi. (C, F, I and L) CI of S. Tm loads in mLN at 8 dpi. Bar indicates median. ns, not significant; *P < 0.05; **P < 0.01; ***P < 0.001; Mann-Whitney U test.