Albumin led to the reduction of intracellular superoxide anion level induced by hydrogen peroxide in mouse mesangial cells.
(A) Representative image of intracellular superoxide anion detected by dihydroethidium (DHE) staining in SV40 MES-13 cells after 6-h exposure to hydrogen peroxide (500 μM) with or without bovine serum albumin (BSA, 3.0 g/dL). As a control (CTR), we used the cells that were not exposed to hydrogen peroxide. (B, C) The DHE fluorescent intensity per cell of SV40 MES-13 cells that were exposed to hydrogen peroxide with or without BSA or with γ-globulin. MES-13 cells were exposed to 500 μM hydrogen peroxide for 6 h with or without BSA (0.75, 1.5, 3.0, and 4.5 g/dL) or with 3.0 g/dL γ-globulin, and the DHE fluorescent intensity per cell was determined by the fluorescent plate reader. As a CTR, we used the cells that were not exposed to hydrogen peroxide. Data are presented as the mean ± standard error of three independent experiments, each of which was repeated at least three times with similar results obtained. * P <0.05 vs. CTR, † P <0.05 vs. H2O2 500 μM, ‡ P <0.05 vs. H2O2 500 μM + γ-globulin.