Adult offspring in vivo and in vitro cardiovascular function.
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Adult offspring in vivo basal: 4A, mean arterial pressure; 4B, systolic pressure; 4C, diastolic pressure; 4D, femoral blood flow; 4E, femoral vascular conductance; and 4F, heart rate. Adult offspring in vivo stimulated femoral blood flow and femoral vascular conductance responses to 4G, PE; 4H, Ang II; and 4I, SNP. 4J. Femoral vascular resistance response after 10 minutes of L-NAME exposure (change from baseline). 4K. In vitro concentration–response curves of the percent Rmax to methacholine. 4L. The NO-dependent, EDHF-dependent, and PG-dependent components (AAC) of vasorelaxation in second-order femoral resistance arteries isolated from 9-month-old adult offspring. Values are mean ± SEM for all data. Groups are N (open symbols, n = 6–11), H (black/grey symbols, n = 6–11), HC (red symbols, n = 5–11), and NC (blue symbols, n = 6–15). Significant (P < 0.05) differences are *versus normoxia, †H versus HC, one- or two-way ANOVA with posthoc Tukey test (4A–4F, 4J and 4L), or two-way repeated measures ANOVA with posthoc Tukey test (4G–4I, 4K). AngII, angiotensin II; AAC, area above the curve; EDHF, endothelium derived hyperpolarising factor; H, hypoxia; HC, hypoxia with vitamin C; L-NAME, N(ω)-nitro-L-arginine methyl ester; N, normoxia; NC, normoxia with vitamin C; PE, phenylephrine; percent Rmax, maximal femoral vessel relaxation; PG, prostaglandin; SNP, sodium nitroprusside.