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Adult offspring in vivo and in vitro cardiovascular function.

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posted on 22.01.2019 by Kirsty L. Brain, Beth J. Allison, Youguo Niu, Christine M. Cross, Nozomi Itani, Andrew D. Kane, Emilio A. Herrera, Katie L. Skeffington, Kimberley J. Botting, Dino A. Giussani

Adult offspring in vivo basal: 4A, mean arterial pressure; 4B, systolic pressure; 4C, diastolic pressure; 4D, femoral blood flow; 4E, femoral vascular conductance; and 4F, heart rate. Adult offspring in vivo stimulated femoral blood flow and femoral vascular conductance responses to 4G, PE; 4H, Ang II; and 4I, SNP. 4J. Femoral vascular resistance response after 10 minutes of L-NAME exposure (change from baseline). 4K. In vitro concentration–response curves of the percent Rmax to methacholine. 4L. The NO-dependent, EDHF-dependent, and PG-dependent components (AAC) of vasorelaxation in second-order femoral resistance arteries isolated from 9-month-old adult offspring. Values are mean ± SEM for all data. Groups are N (open symbols, n = 6–11), H (black/grey symbols, n = 6–11), HC (red symbols, n = 5–11), and NC (blue symbols, n = 6–15). Significant (P < 0.05) differences are *versus normoxia, †H versus HC, one- or two-way ANOVA with posthoc Tukey test (4A–4F, 4J and 4L), or two-way repeated measures ANOVA with posthoc Tukey test (4G–4I, 4K). AngII, angiotensin II; AAC, area above the curve; EDHF, endothelium derived hyperpolarising factor; H, hypoxia; HC, hypoxia with vitamin C; L-NAME, N(ω)-nitro-L-arginine methyl ester; N, normoxia; NC, normoxia with vitamin C; PE, phenylephrine; percent Rmax, maximal femoral vessel relaxation; PG, prostaglandin; SNP, sodium nitroprusside.