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Additional file 9: of The lncRNA MIR2052HG regulates ERα levels and aromatase inhibitor resistance through LMTK3 by recruiting EGR1

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posted on 03.04.2019, 05:00 by Junmei Cairns, James Ingle, Krishna Kalari, Lois Shepherd, Michiaki Kubo, Matthew Goetz, Richard Weinshilboum, Liewei Wang
Figure S7. EGR1, but not MIR2052HG, regulates LMTK3 expression in HER2+ and TNBC cells. a–b Cell proliferation of HER+ Au565 (a) and TNBC MDA-MB-231 (b) cells after knocking down LMTK3. LMTK3 gene expression and EGR1 knockdown efficiency was determined by qRT-PCR. c–d ChIP analysis demonstrates the binding of EGR1 to the promoter region of the LMTK3 gene locus in AU565 (c) and MDA-MB-231 (d) cells. However, knockdown of MIR2052HG did not change the binding. IgG serves as a control. Error bars represent SEM of three independent experiments in triplicate; **p < 0.01, Non-significant (NS): p > 0.05. (TIF 1117 kb)