Additional file 6: of p53 mutant-type in human prostate cancer cells determines the sensitivity to phenethyl isothiocyanate induced growth inhibition
figureposted on 15.07.2019 by Monika Aggarwal, Rahul Saxena, Nasir Asif, Elizabeth Sinclair, Judy Tan, Idalia Cruz, Deborah Berry, Bhaskar Kallakury, Quynhchi Pham, Thomas Wang, Fung-Lung Chung
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
Figure S6. PEITC inhibits growth in a p73-independent manner. DU145 cells transfected with HA-p73α-pcDNA3 or pcDNA3 were treated with PEITC for 24 h. (a) Percent cell proliferation was determined by the WST-1 assay, and (b) Apoptosis was measured by Annexin-V staining by flow cytometry using a BD LSRFORTESSA instrument. Left Panel; representative pictures of flow cytometry data, Right Panel; quantification of the data. (***p ≤ 0.0000 and *p ≤ 0.02). (c) DU145 cells transfected with HA-p73α-pcDNA3 were treated with DMSO or the indicated concentration of PEITC for 4 h. Blots were probed with anti-p73 and anti-p53 (p53 DO-1) antibodies and reprobed with anti-GAPDH antibody. (d) qRT-PCR of p21 gene in DU145 cells transfected with HA-p73α-pcDNA3 or pcDNA3 and treated with PEITC for 4 h. Results are expressed as ± SD. (***p ≤ 0.0000 and **p ≤ 0.002). (TIF 602 kb)