13046_2019_1267_MOESM6_ESM.tif (602.82 kB)

Additional file 6: of p53 mutant-type in human prostate cancer cells determines the sensitivity to phenethyl isothiocyanate induced growth inhibition

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posted on 15.07.2019, 05:00 by Monika Aggarwal, Rahul Saxena, Nasir Asif, Elizabeth Sinclair, Judy Tan, Idalia Cruz, Deborah Berry, Bhaskar Kallakury, Quynhchi Pham, Thomas Wang, Fung-Lung Chung
Figure S6. PEITC inhibits growth in a p73-independent manner. DU145 cells transfected with HA-p73α-pcDNA3 or pcDNA3 were treated with PEITC for 24 h. (a) Percent cell proliferation was determined by the WST-1 assay, and (b) Apoptosis was measured by Annexin-V staining by flow cytometry using a BD LSRFORTESSA instrument. Left Panel; representative pictures of flow cytometry data, Right Panel; quantification of the data. (***p ≤ 0.0000 and *p ≤ 0.02). (c) DU145 cells transfected with HA-p73α-pcDNA3 were treated with DMSO or the indicated concentration of PEITC for 4 h. Blots were probed with anti-p73 and anti-p53 (p53 DO-1) antibodies and reprobed with anti-GAPDH antibody. (d) qRT-PCR of p21 gene in DU145 cells transfected with HA-p73α-pcDNA3 or pcDNA3 and treated with PEITC for 4 h. Results are expressed as ± SD. (***p ≤ 0.0000 and **p ≤ 0.002). (TIF 602 kb)


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