13045_2020_1029_MOESM6_ESM.tif (2.13 MB)
Download file

Additional file 6 of STOML2 potentiates metastasis of hepatocellular carcinoma by promoting PINK1-mediated mitophagy and regulates sensitivity to lenvatinib

Download (2.13 MB)
figure
posted on 15.01.2021, 04:27 by Yahui Zheng, Chong Huang, Lu Lu, Kangkang Yu, Jing Zhao, Mingquan Chen, Lu Liu, Qingfeng Sun, Zhifei Lin, Jianming Zheng, Jinhong Chen, Jubo Zhang
Additional file 6: Figure S4. Lenvatinib induces cytoprotective mitophagy in HCC cells. (A) Double fluorescence staining of mitochondria by JC-1 was applied to monitor the mitochondrial membrane potential (MMP), as green fluorescent J-monomers indicating loss of MMP, and red fluorescent J-aggregates reflecting higher MMP. The MMP of SMMC-7721 and HCCLM3 with lenvatinib (10 μM, 4 h) treatment decreased significantly compared with controls by flow cytometric analysis. (B-C) Confocal microscopy was performed to detect spatial colocalization of mitochondrial protein COXIV (red) and PINK1 (green) (B) or TOMM20 (red) and LAMP1 (green) (Scale bars:10 μm) (C) in HCCLM3 cells with or without the treatment of lenvatinib (10 μM, 24 h). (Scale bars:10 μm) **P<0.01; ***P<0.001; ns, no significance.

Funding

National Natural Science Foundation of China (CN) National Natural Science Foundation of China Natural Science Foundation of Shanghai Zhejiang Province Public Welfare Technology Application Research Project National Key Scientific Instrument and Equipment Development Projects of China

History