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Additional file 6: Figure S6. of ROCK activity and the Gβγ complex mediate chemotactic migration of mouse bone marrow-derived stromal cells

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posted on 2015-07-24, 05:00 authored by Caroline Ryan, James Brown, Emer Bourke, Áine Prendergast, Claire Kavanagh, Zhonglin Liu, Peter Owens, Georgina Shaw, Walter Kolch, Timothy O’Brien, Frank Barry
Inhibitors do not affect BMSC viability or cytotoxicity or induce apoptosis; 24-h serum starvation (SFM) of BMSCs alone or in conjunction with 10 μM Y27632, 5 μM LY294002, 10 μM MPS-Phos, or 0.5 μM Staurosporine. A very low dose of Staurosporine was included as a positive control for apoptosis. Untreated cells (cultured in normal media) were included as an untreated control. SFM set = 1. Graph represents three independent experiments. (Each individual experiment was performed in triplicate.) Data are presented as mean ± standard error of the mean. A P value of less than 0.05 (*) was deemed significant, and a P value of less than 0.01 (**) very significant. BMSC bone marrow-derived stromal cell, SFM serum-free medium. (PNG 360 kb)