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Additional file 5 of STOML2 potentiates metastasis of hepatocellular carcinoma by promoting PINK1-mediated mitophagy and regulates sensitivity to lenvatinib

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posted on 15.01.2021, 04:26 by Yahui Zheng, Chong Huang, Lu Lu, Kangkang Yu, Jing Zhao, Mingquan Chen, Lu Liu, Qingfeng Sun, Zhifei Lin, Jianming Zheng, Jinhong Chen, Jubo Zhang
Additional file 5: Figure S3. STOML2 promotes mitophagy in HCC cells under stress. (A–B) Confocal microscopy was performed to detect spatial colocalization of mitochondrial protein COXIV (red) and PINK1 (green) in SMMC-7721 and HCCLM3 control and derived cells under the treatment of CCCP (10 μM) for 4 h. (Scale bars:10 μm) (C) mRNA levels of STOML2 and PINK1 were determined by qRT-PCR in SMMC-7721 and HCCLM3 with manipulated the expression of STOML2, taking GAPDH mRNA as a control. (D) The alteration of PINK1 protein in SMMC-7721 and HCCLM3 control and derived cells with co-treatment of 10μM CCCP and 20 μM MG132 was detected by Western blot. **P<0.01; ***P<0.001; ns, no significance.

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National Natural Science Foundation of China (CN) National Natural Science Foundation of China Natural Science Foundation of Shanghai Zhejiang Province Public Welfare Technology Application Research Project National Key Scientific Instrument and Equipment Development Projects of China

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