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Additional file 5: Figure S4. of Sortin2 enhances endocytic trafficking towards the vacuole in Saccharomyces cerevisiae

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posted on 25.07.2015, 05:00 authored by Beatriz Vásquez-Soto, Nicolás Manríquez, Mirna Cruz-Amaya, Jan Zouhar, Natasha Raikhel, Lorena Norambuena
Figure 3. Enhancing of endocytic trafficking depends on Sortin2 structural features. S. cerevisiae parental line was grown on YPD 1% DMSO (control) and YPD supplemented with 20 μM of different Sortin2-structural analogs. Afterwards cells were incubated with 24 μM FM4-64 for 30 min at 4°C. Then turned to 28°C to be imaged subsequently by confocal microscopy at different incubation times. Images of twenty-five min incubation are shown. Two images are representative of 20 cells. The experiment was performed more than 3 times. Scale bar represents 5 μm.