Additional file 4 of Genome-wide screen identifies new set of genes for improved heterologous laccase expression in Saccharomyces cerevisiae
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posted on 2024-08-14, 20:09 authored by Garrett Strawn, Ryan W K Wong, Barry P Young, Michael Davey, Corey Nislow, Elizabeth Conibear, Christopher J R Loewen, Thibault MayorSupplementary Material 4
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Canada Foundation for Innovation Natural Sciences and Engineering Research Council of Canada
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yeast saccharomyces cerevisiaeyeast knockout collectiontyrosine phosphatase genesaccharomyces cerevisiae abstractprovides new avenuesone major drawbackgene ontology analysisski complex responsibleassociated degradation pathwayproduced proteins comparedkey chaperone proteinser quality controler dnaj coincluding many genes200 gene deletionssecreted laccase activityrelatively low yieldcomplex proteinssecreted laccaseoverall yieldmrna degradationrecombinant proteinsmitochondrial proteinswidely usedvesicle traffickingspecifically focusingsignificant portionsecrete largeprecise dosingoverlay assaymutants revealedmannosyltransferases involvedliquid cultureidentify mutantshost systemshost cellglycolipid metabolismfast growtheffective culturingdetrimental effectcopy plasmidalso identified
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