12885_2018_4464_MOESM3_ESM.tif (9.46 MB)

Additional file 3: of Histone methyltransferase SETDB1 promotes cells proliferation and migration by interacting withTiam1 in hepatocellular carcinoma

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posted on 08.05.2018, 05:00 by Yuqin Zhang, Jing Huang, Qisheng Li, Keli Chen, Yonghao Liang, Zetao Zhan, Feng Ye, Wen Ni, Longhua Chen, Yi Ding
Figure S2. (A) Expression of the BD-hTiam1-C1199 clones detected by anti-c-Myc antibody. (ladder1:BD alone, ladder1 2: BD-p53 fusion protein, ladder3-5: BD-hTiam1-C1199 clone #1-3). (B)Testing for autoactivation by plating AH109 cells co-transformed with the bait plasmid and the AD vector on SCM-2 and -3 plates. SCM-2, SCM plate lacking leucine and tryptophan; cell growth shows the successful co-transformation; SCM-3, SCM plate lacking leucine, tryptophan and histidine; no cell growth shows no autoactivation of transcription by the BD-hTiam1-C1199 fusion protein. (a. SCM/−Trp-Leu (− 2) Plate;b. SCM/−Leu-Trp-His (− 3) Plate). (TIF 9682 kb)