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Additional file 3: of Histone methyltransferase SETDB1 promotes cells proliferation and migration by interacting withTiam1 in hepatocellular carcinoma

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posted on 08.05.2018 by Yuqin Zhang, Jing Huang, Qisheng Li, Keli Chen, Yonghao Liang, Zetao Zhan, Feng Ye, Wen Ni, Longhua Chen, Yi Ding
Figure S2. (A) Expression of the BD-hTiam1-C1199 clones detected by anti-c-Myc antibody. (ladder1:BD alone, ladder1 2: BD-p53 fusion protein, ladder3-5: BD-hTiam1-C1199 clone #1-3). (B)Testing for autoactivation by plating AH109 cells co-transformed with the bait plasmid and the AD vector on SCM-2 and -3 plates. SCM-2, SCM plate lacking leucine and tryptophan; cell growth shows the successful co-transformation; SCM-3, SCM plate lacking leucine, tryptophan and histidine; no cell growth shows no autoactivation of transcription by the BD-hTiam1-C1199 fusion protein. (a. SCM/−Trp-Leu (− 2) Plate;b. SCM/−Leu-Trp-His (− 3) Plate). (TIF 9682 kb)

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