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Additional file 3: of Comparison of gene expression profile of the spinal cord of sprouting-capable neonatal and sprouting-incapable adult mice

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posted on 31.07.2019, 03:59 by Hiroshi Tsujioka, Toshihide Yamashita
Negative control of in situ hybridization for Ly86 and Mpeg1. Representative images of negative controls for Figs. 7 and 8 using sense probes for Ly86 (A-D) or Mpeg1 (E-H) are shown. The spinal cord of sham (A, C, E, G) or pyramidotomy (B, D, F, H) group which were injured at P7 (A, B, E, F) or 8 W (C, D, G, H) are shown. Right sides of the spinal cords are marked with black pigment. Blue/purple color is non-specific binding of sense probe. Dorsal is at the top, ventral is bottom, left is to the left, right is to the right. Scale bars: 200 μm. Because floating slices shrank during hybridization, the edge of the slices were folded in most cases when they were mounted on glass slides. (TIF 9508 kb)


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