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Additional file 3 of Circ-MBOAT2 knockdown represses tumor progression and glutamine catabolism by miR-433-3p/GOT1 axis in pancreatic cancer

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posted on 09.04.2021, 03:22 by Xiaoxiao Zhou, Kun Liu, Jing Cui, Jiongxin Xiong, Heshui Wu, Tao Peng, Yao Guo
Additional file 3: Figure S3. Circ-MBOAT2 overexpression abolished si-circ-MBOAT2-mediated pancreatic cancer cell processes. (A-J) PANC-1 and SW1990 cells were transfected with si-NC, si-circ-MBOAT2, si-circ-MBOAT2 + pCD5-ciR and si-circ-MBOAT2 + circ-MBOAT2, respectively. (A) Circ-MBOAT2 expression was detected by qRT-PCR. (B-D) Cell viability and colony-forming ability were revealed by MTT and cell colony formation assays, respectively. (E) Flow cytometry analysis was used to determine cell apoptosis. (F and G) The invasion and migration of cells were demonstrated by transwell invasion and wound-healing assays, respectively. (H and I) Glutamine and α-KG assay kits were utilized to reveal glutamine consumption and α-KG production. (J) Glutamate assay kit was performed to detect glutamate production. **P < 0.01, ***P < 0.001 and ****P < 0.0001.

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