Additional file 3: Figure S3. of Erratum to: Type IV collagen drives alveolar epithelial–endothelial association and the morphogenetic movements of septation
figureposted on 24.10.2019 by Maria Loscertales, Fotini Nicolaou, Marion Jeanne, Mauro Longoni, Douglas Gould, Yunwei Sun, Faouzi Maalouf, Nandor Nagy, Patricia Donahoe
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
(A–D) Histology of R26-Cre ER ; Col4a1 +/Flex41 and Tie2-Cre; Col4a1 +/Flex41 mutants, and (E–I) immunohistochemistry analysis of PDPL-pSPC, HOPX, α-SMA, tropoelastin, and elastin in R26-Cre ER ; Col4a1 +/Flex41 . (A, B) Hematoxylin and eosin shows that both R26-Cre ER ; Col4a1 +/Flex41 and Tie2-Cre; Col4a1 +/Flex41 mutants have simplified alveolarization. (C) R26-Cre ER ; Col4a1 +/Flex41 septa are thick and with numerous blood capillaries (arrowheads) and cells with lipid content (arrows). (D) Tie2-Cre; Col4a1 +/Flex41 septa are small and short with increases in blood capillaries (arrowheads), but not in cells with lipid content. (E) pSPC and PDPL co-staining shows a disorganized alveolar epithelium. (F) R26-Cre ER ; Col4a1 +/Flex41 display a decrease of type I pneumocytes as shown by nuclear staining of HOPX. (G–I) Abnormal localization of α-SMA, tropoelastin and elastin in the septa of R26-Cre ER ; Col4a1 +/Flex41 lungs. Scale bars = 200 μm in A and B, 35 μm in C and D, 50 μm in E, and 100 μm in F to I. (TIF 24706 kb)