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Additional file 3: Figure S3. of A puromycin selectable cell line for the enrichment of mouse embryonic stem cell-derived V3 interneurons

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posted on 10.11.2015 by Hao Xu, Nisha Iyer, James Huettner, Shelly Sakiyama-Elbert
Physiological parameters associated with spiking phenotypes. (A) Rheobase (current required to reach threshold) plotted versus input resistance for cells from days 2–5 (squares) or days 6–13 (circles) that fired single (open), adapting (grey), or multiple (black) action potentials during an 800 msec current pulse. Input resistance decreased with time after selection, but did not correlate with spiking phenotype. Cells from days 6–13 firing single action potentials had a significantly higher rheobase (P <0.01, one-way ANOVA on ranks). (B) Steady-state outward current density (I K density) versus peak inward current density (I Na density) at 0 mV. Single-spiking cells had the lowest mean inward current density and the second highest outward current density but the differences were not significant. (C) Current density ratio (I Na/I K) versus threshold. Repetitive firing cells from days 6–13 had a significantly lower threshold (P <0.002, one-way ANOVA). Single-spiking cells had the lowest mean current density ratio, although the differences were not significant. (JPG 447 kb)


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