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Additional file 2: of Reduction in sucrose contents by downregulation of fructose-1,6-bisphosphatase 2 causes tiller outgrowth cessation in rice mutants lacking glutamine synthetase1;2

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posted on 22.12.2018, 05:00 authored by Miwa Ohashi, Keiki Ishiyama, Miyako Kusano, Atsushi Fukushima, Soichi Kojima, Toshihiko Hayakawa, Tomoyuki Yamaya
Figure S2. In situ hybridization of OscFBP1 in the basal portions of the shoots. Longitudinal sections of the basal portions of the shoots were prepared from wild-type (WT) (a-c, g-i) and gs1;2 mutant (gs1;2) (d-f) rice grown hydroponically in the presence of 1 mM NH4Cl until the fourth leaf stage. The antisense probe for the OscFBP1 transcript was hybridized with the longitudinal sections from the shoot basal portions of wild-type (a-c) and gs1;2 mutant (d-f) rice. The sense probe for the OscFBP1 transcript was hybridized with the sections from the shoot basal portions of the wild type (g-i) as a negative control. The phloem companion cells of the nodal vascular anastomoses (a, d, g), the internodal parenchyma cells (b, e, h), and the leaf sheath (c, f, i) are shown. The red arrowhead in (a, d) indicates the hybridization signal of the OscFBP1 transcript in the phloem companion cells of the nodal vascular anastomoses. Abbreviation: cc, companion cell. Scale bars: 20 μm (a, b, d, e, g, h) and 50 μm (c, f, i). (TIF 5981 kb)

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