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Additional file 2: of Nodal induces apoptosis and inhibits proliferation in ovarian endometriosis-clear cell carcinoma lesions

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posted on 03.04.2019 by Rinako Miura, Ako Yokoi, Toshihide Matsumoto, Yasuko Oguri, Miki Hashimura, Masataka Tochimoto, Sabine Kajita, Makoto Saegusa
Figure S2. Overexpression of Nodal enhances susceptibility to apoptosis in OEmCa cells. (A) After treatment of the stable Nodal-expressing Ish-NoD and mock cells with 1 μg/mL Dox for the times indicated, cells undergoing apoptosis (sub-G1) were detected by flow cytometry. This experiment was performed in triplicate using independent samples. (B) Upper: after treatment with 1 μg/mL Dox, the stable Nodal-expressing Ish-NoD and mock cells undergoing apoptosis (indicated by arrows) were detected by TUNEL assay. Original magnification, × 400. Lower: number of apoptotic cells per 10 high power fields (HPFs) detected by TUNEL assay for the times shown. (C) Left: western blot analysis of the indicated proteins in the stable Nodal-expressing Ish-NoD and mock cells after 1 μg/mL Dox treatment for the times shown. Right: values of endogenous Bcl2 relative to Bax protein were calculated by normalization to β-actin in the stable Nodal-expressing Ish-NoD and mock cells after 1 μg/mL Dox treatment for the times shown. (TIF 3448 kb)

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