Additional file 2: of MoPer1 is required for growth, conidiogenesis, and pathogenicity in Magnaporthe oryzae

Figure S2. Targeted gene replacement and complementation. (A) A 1384-bp fragment of the MoPER1 coding region were replaced by a 1.4-kb fragment containing the hygromycin B resistance cassette to create MoPER1 deletion mutant. (B) Southern hybridization analysis was used to validate the deletion of MoPER1 gene and the addition of a single copy integration of the HPH gene. (C) Semiquantitative RT-PCR was carried out to confirm the deletion and reintroduction of MoPER1 gene. (TIF 247 kb)