13395_2015_69_MOESM2_ESM.tif (1.97 MB)

Additional file 2: Figure S1. of Genetic disruption of Ano5 in mice does not recapitulate human ANO5-deficient muscular dystrophy

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posted on 2015-12-21, 05:00 authored by Jing Xu, Mona El Refaey, Li Xu, Lixia Zhao, Yandi Gao, Kyle Floyd, Tallib Karaze, Paul Janssen, Renzhi Han
Test of anti-Ano5 antibodies by Western blotting. a The anti-Ano5 antibody (sc-169626) from Santa Cruz Biotechnology did not detect any positive signal in human Ano5-expressing cell lysate, which was correctly detected by the anti-GFP antibody at the predicted size. b The anti-Ano5 antibody (AP8580B) from Abgent produced a prominent band at about 100 kDa in both negative and positive samples; however, this band is not Ano5 because the Ano5-YFP fusion protein should be around 135 kDa as correctly detected by the anti-GFP antibody. The arrows point to the correct Ano5-YFP fusion protein. These experiments were repeated at least three times.


National Heart, Lung, and Blood Institute