Additional file 1 of Transcriptomic and metabolomic analyses reveal the essential nature of Rab1B in Toxoplasma gondii

Additional file 1. Depletion of Rab1B caused disorganized endomembrane in parasites. The iRab1B parasites were grown in ATc for 18 and 24 h, followed by fixation, embedding and slicing for transmission electron microscopy (TEM). Normal endomembranes were observed in the TATi line (a), while defects of the endomembrane were observed in the iRab1B induced by ATc for 18h (b). The parasites with normal or abnormal endomembranes were quantified in the TATi and iRab1B lines when Rab1b was treated with ATc for 0, 18 and 24 h, respectively (c). Scale bar = 200 nm.