Additional file 1 of Analysis of neurexin-neuroligin complexes supports an isoform-specific role for beta-neurexin-1 dysfunction in a mouse model of autism

Supplementary Material 1: Suppl. Figure 1 Surface expression of Flag-tagged Nrxn1 constructs. Description of data: Images show HEK293T cells transfected with alpha-Nrxn1 (-AS4) and beta-Nrxn1 (-AS4) either containing or lacking a Flag tag at the cytoplasmic domain, as indicated. Cells were surface-stained with anti HA antibodies in non-permeabilizing conditions, followed by permeabilization and incubation with an anti-Flag antibody. All constructs contain an HA epitope at the N-terminus of the extracellular domain. Surface staining was not affected by the insertion of a Flag tag in Nrxn1. Nuclei were stained with DAPI. Scale bar, 5 μm.