Additional file 1: Figure S1. of MITF and PU.1 inhibit adipogenesis of ovine primary preadipocytes by restraining C/EBPβ
figureposted on 17.01.2017 by ChongMei Ruan, Xiu Li, JunJie Hu, Yong Zhang, XingXu Zhao
Figures are generally photos, graphs and static images that would be represented in traditional pdf publications.
Overexpression of MITF and its co-activator PU.1 inhibited the adipogenesis of ovine primary preadipocytes. On reaching 80% confluence, the primary cells were transfected with pcDNA-MITF, pcDNA-PU.1, pcDNA-MITF and pcDNA-PU.1, or nothing. Then the cells were induced to differentiate. On day 2 of adipogenic differentiation, their total proteins and RNA were extracted. A, B – Protein levels of MITF, PU.1, C/EBPβ, PPARγ and aP2 after pcDNA-MITF and/or pcDNA-PU.1 transfection. C, D – mRNA levels of MITF, PU.1, C/EBPβ, PPARγ and aP2 after pcDNA-MITF and/or pcDNA-PU.1 transfection. Significance was set at p < 0.05 (n = 3), *p < 0.05 vs. control, # p < 0.05 vs. pc-MITF. (TIF 462 kb)