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Additional file 11 of Clinical recovery of Macaca fascicularis infected with Plasmodium knowlesi

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posted on 2021-12-31, 04:10 authored by Mariko S. Peterson, Chester J. Joyner, Jessica A. Brady, Jennifer S. Wood, Monica Cabrera-Mora, Celia L. Saney, Luis L. Fonseca, Wayne T. Cheng, Jianlin Jiang, Stacey A. Lapp, Stephanie R. Soderberg, Mustafa V. Nural, Jay C. Humphrey, Allison Hankus, Deepa Machiah, Ebru Karpuzoglu, Jeremy D. DeBarry, Rabindra Tirouvanziam, Jessica C. Kissinger, Alberto Moreno, Sanjeev Gumber, Eberhard O. Voit, Juan B. Gutiérrez, Regina Joice Cordy, Mary R. Galinski
Additional file 11: Fig. S2. E33 Experimental Design and Parasitaemia, as also similarly presented in PlasmoDB [91] to accompany E33 publicly available data. E33 includes iterative P. knowlesi infections in a cohort of four kra monkeys (13C90, 14C15, 14C3, H13C110) to continue to study acute and early stage “Chronic I” infections. Top Panel: Schematic of the planned (generalized) experimental design with timepoints (TP) of sample collection (gold bars), P. knowlesi cryopreserved sporozoite inoculations at day 0 (syringe), predicted parasitaemia kinetics (pink line) with early infection, log-phases, peaking parasitaemia, and later TPs indicated for blood and bone marrow sample collections. Necropsy endpoints (*) were planned for each species after day 14, early on in the natural overall decline in parasitaemia as observed for kra monkeys in E07 (Fig. S1) and again subsequently in E35 (Fig. S3). Bottom Panel: Schematic showing E33 experimental data including P. knowlesi cryopreserved sporozoite inoculations on day 0 (syringe), daily parasitaemias graphed (pink lines), and defined TPs (gold bars) and the specific days of euthanasia and necropsy endpoints (*) are indicated for each of the animals. No treatment was required.


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