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Acute RB loss accelerates UV-induced DNA damage repair

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posted on 30.12.2011, 14:56 authored by Emily E. Bosco, Erik S. Knudsen

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Taken from "Differential role of RB in response to UV and IR damage"

Nucleic Acids Research 2005;33(5):1581-1592.

Published online 14 Mar 2005


© The Author 2005. Published by Oxford University Press. All rights reserved

() To examine the role of RB in the kinetics of UV-induced CPD and 6-4 PP lesion repair, Ad-GFP and Ad-GFP-Cre infected MAFs were treated with 0 or 10 J/m UV and harvested for genomic DNA purification at 0, 5 and 10 h. Increasing concentrations of denatured genomic DNA were vacuum blotted onto a membrane and probed with anti-CPD and anti-6-4 PP antibodies (top panel). The lesion intensities in the top panel were quantified using Metmorph and plotted as percent reduction in lesion intensity from cells treated with 10 J/m UV and harvested at = 0 to those harvested at the indicated timepoint (bottom panel). () As performed in (A), cells were treated with UV and cultured prior to harvesting at 0 and 24 h. Dot blot analysis was performed as previously described to confirm previous reports of impaired repair of CPD lesions in murine cells and to reveal the effect of acute RB loss on acceleration of 6-4 PP repair.