pgen.1009452.g006.tif (2.86 MB)

Activity of Ppn1 truncations in CTD mutant backgrounds in which Ppn1 is essential.

Download (2.86 MB)
posted on 2021-03-12, 18:39 authored by Bradley Benjamin, Ana M. Sanchez, Angad Garg, Beate Schwer, Stewart Shuman

S. pombe strains bearing the indicated ppn1 truncation alleles in combination with rpb1-CTD phospho-site mutants were spot-tested for growth on YES agar at the indicated temperatures. The ppn1-(1–496) allele, which did not complement the cs growth phenotype seen in ppn1Δ, was lethal in the rpb1-Y1F, -S2A, and -T4A backgrounds.