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Download fileActivity of Ppn1 truncations in CTD mutant backgrounds in which Ppn1 is essential.
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posted on 2021-03-12, 18:39 authored by Bradley Benjamin, Ana M. Sanchez, Angad Garg, Beate Schwer, Stewart ShumanS. pombe strains bearing the indicated ppn1 truncation alleles in combination with rpb1-CTD phospho-site mutants were spot-tested for growth on YES agar at the indicated temperatures. The ppn1-(1–496) allele, which did not complement the cs growth phenotype seen in ppn1Δ, was lethal in the rpb1-Y1F, -S2A, and -T4A backgrounds.
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CPFlncRNA-mediated transcriptional int...Swd 22 Fission yeast Cleavagethree-subunit DPS sub-complex710- aa Ppn 1 proteinPpn 1 functionN-terminal 172- aaPpn 1fission yeast cleavagePPdis 2 Δ cellsPpn 1 activityPpn 1 aa 506Ppn 1 truncationsSwd 22 interactionPpn 1 aa 533RNA polymerase IIyeast two-hybrid assaysdeletion alleviated hypomorphic phe...Dis 2subunit Ppn 1DPS Δ down-regulated phosphate home...Ppn 1 subunitDis 2 interaction-defective missens...
