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Activity of Ppn1 truncations in CTD mutant backgrounds in which Ppn1 is essential.

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posted on 12.03.2021, 18:39 by Bradley Benjamin, Ana M. Sanchez, Angad Garg, Beate Schwer, Stewart Shuman

S. pombe strains bearing the indicated ppn1 truncation alleles in combination with rpb1-CTD phospho-site mutants were spot-tested for growth on YES agar at the indicated temperatures. The ppn1-(1–496) allele, which did not complement the cs growth phenotype seen in ppn1Δ, was lethal in the rpb1-Y1F, -S2A, and -T4A backgrounds.

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