posted on 2011-12-30, 16:55authored byYakhya Dieye, Jessica L Dyszel, Rebin Kader, Brian MM Ahmer
Copyright information:
Taken from "Systematic analysis of the regulation of type three secreted effectors in serovar Typhimurium"
BMC Microbiology 2007;7():3-3.
Published online 18 Jan 2007
PMCID:PMC1781944.
The expression of the operon under the control of the Ppromoter was analyzed in the wild type and the isogenic mutant and mutant with or without complementation. , the strains were grown in MgM or in LB for six hours, and the light emitted by bacterial suspensions of similar cell density were captured using the Xenogen IVIS imaging system (Alameda, CA) and displayed as pseudocolor images with blue representing the lowest and red the highest light intensity. 1, wild type + P; 2, wild type + pSB384 vector control; 3, + P; 4, + P; 5, + P+ ; 6, + P+ pWSK29 control. , quantification of the activation of the Ppromoter. The light emitted by 100 microliters of culture was measured using a luminometer. The values represent the mean ± SEM of three independent experiments.