A genome-wide imaging based siRNA screen identifies regulators of membrane-associated E-cadherin in SW480 colon cancer cells.
(A) Membrane associated Ecad scores normalised to mock transfectants for all SMARTpool siRNAs (black) transfected into SW480 cells. Controls are highlighted: mock (blue), siZEB1 (orange), siCDH1 (red) & siPLK1 (green). (B) Mock normalised Robust Z-score (Ecad) plot for SMARTpool siRNA screen. The cut-off for E-cadherin negative regulatory genes is indicated by the red-dotted line (Z-score>5.16); the Z- score cut-off for positive regulatory genes is <0.036. (C) Normalised Robust Z-score (Ecad) (Z-score Ecad) is shown for genes with a functional association with E-cadherin regulation and a gene* with potential miRNA-200 family off-target effects.