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A dual library screening strategy for the identification of targets responsible for observed phenotypes

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posted on 31.12.2011, 07:18 by Scott E. Martin, Tamara L. Jones, Cheryl L. Thomas, Philip L. Lorenzi, Dac A. Nguyen, Timothy Runfola, Michele Gunsior, John N. Weinstein, Paul K. Goldsmith, Eric Lader, Konrad Huppi, Natasha J. Caplen

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Taken from "Multiplexing siRNAs to compress RNAi-based screen size in human cells"

Nucleic Acids Research 2007;35(8):e57-e57.

Published online 28 Mar 2007

PMCID:PMC1885663.

© 2007 The Author(s)

() The identified members of active siRNA multiplexes affecting cell viability. These target genes were identified by cross-referencing the components of the top 20 down-regulating multiplexes within library #1 with common components found in the top 20 down-regulators within library #2. () Validation of the identified targets as down-regulators of cell viability in an independent assay. All values are reported as the percent viability of cells transfected with a negative-control siRNA. As an additional reference, a multiplex found to approximate the median value in all three screens of library #1 was also used. () Evaluation of both siRNAs against the top five gene targets individually. The experiment was performed as described in (). The activity of siRNA-1 did not differ from that of the median multiplex. For each target, the activity of siRNA pairs was approximately equal to that of the more active siRNA (compare () with ()).

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