A/SLOV15 S13 displays enhanced receptor binding to α-2,6 receptor analogues relative to A/SLOV15 V1.

A/SLOV15 V1 and A/SLOV15 S13 receptor analogue binding was assessed by biolayer-interferometry (BLI) using the Octet RED96 system and MM/GBSA analysis. (A,B) BLI receptor binding assays included 6.8 Log₁₀ HA vRNA copies of each purified virus and binding is expressed as fractional saturation as a function of sugar loading with a best non-linear least-squares fit to the Hill equation. (A) Binding of A/SLOV15 V1 (blue) and A/SLOV15 S13 (purple) to an α-2,3 receptor analogue (3-SLN) using the Octet RED96 system. (B) Binding of A/SLOV15 V1 and A/SLOV15 S13 to an α-2,6 receptor analogue (6-SLN). (C) Binding energies of the 3-SLN and 6-SLN receptor analogues with A/SLOV15 V1 and A/SLOV15 S13 were estimated by MM/GBSA analysis (C).