A Rapid and Low-Cost PCR Thermal Cycler for Infectious Disease Diagnostics - Fig 6

(a) Fluorescent signal from reactions using plastic tubes. The PCR tubes that had undergone 45 cycles of TTC RT-PCR amplification were visualized using a cell phone camera. The top two tubes are HIV positive samples, while the bottom two tubes are the same mix that did not go through the RT-PCR process. (b) Gel electrophoresis data of HIV RNA amplicons after RT-PCR using thin-walled polypropylene tubes. Lane 1: ladder. Lanes 2 and 3: commercial RT-PCR (50 cycles in 83 min). Lanes 4 and 5: TTC 330s/10s/45x(15s/30s) (41 min). Lanes 6 and 7: TTC 330s/20s/45x(15s/30s) (41.3 min). (c) Real-time PCR data from the commercial thermal cycler (Cq = 34.8).