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AQP4 immunolabeling of the normal chick retina plus negative controls

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posted on 30.12.2011, 20:03 authored by Melinda J Goodyear, Barbara M Junghans, Loretta Giummarra, Melanie J Murphy, David P Crewther, Sheila G Crewther

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Taken from "A role for aquaporin-4 during induction of form deprivation myopia in chick"

Molecular Vision 2008;14():298-307.

Published online 08 Feb 2008

PMCID:PMC2254964.

Confocal micrograph (40X magnification) with red AQP4 immunolabeling (to the right) of a transverse section of a normal chick retina merged (in middle) with the accompanying differential interference contrast image (to the left) is shown. The layers of the retina are indicated: NFL nerve fiber layer, GC ganglion cell layer, IPL inner plexiform layer, INL inner nuclear layer, IS inner segments of photoreceptors, OS outer segments of photoreceptors, RPE retinal pigment epithelium. It can be seen that labeling is most intense within the NFL, GC and IPL layers. Only very weak label is visible in the INL and photoreceptor region. : Negative control with the primary antibody omitted indicates very weak red non-specific labeling (to the right) merged with the accompanying differential interference contrast image (to the left). : Tissue pre-incubated with purified peptide before AQP4 labeling is shown indicating that blocking of AQP4 sites with the purified peptide successfully prevents binding of the introduced AQP4 antibodies and apparent red immunolabel. Bars=50 μm.

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