AMPK and Ca2+/calmodulin (CaM)-dependent protein kinase kinases (CaMKK2) contribute to the ghrelin activation of NPY neurons.
figureposted on 2017-09-06, 17:41 authored by Hiroshi Hashiguchi, Zhenyu Sheng, Vanessa Routh, Volodymyr Gerzanich, J. Marc Simard, Joseph Bryan
(A) AICAR (400 μM), an AMPK activator, mimics the effect of ghrelin. (B) Compound-C (30 μM), an inhibitor of AMPK, completely blocks the effect of oligomycin. (C) STO-609 (1 μM), a CAMKK2 inhibitor, partially reverses the effect of ghrelin. Ghrelin was applied at 100 pM (red bars). Traces are mean values from 5–20 cells.
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CNQXTTA-PNPY-GFP neuronsSUR 1 agonistNPY GFP-labeled neuronsTrpm 4 antagonistsTrpm 4 modulatorsTTXpresynaptic action potentialsPKAKir 6.2ECAMPKghrelin actionMPNPY neuronsSUR 1growth hormone secretagogue receptorSUR 1 antagonistConclusions NPY neuronsNPY neurons ObjectivesPLCSNX 482-sensitive R-type voltagemeasure ion channel activityIRResults Acylated ghrelin depolarizedGHS-RGABA