figshareBrowse
Fig 4.tif (371.02 kB)
Download file

AMPK and Ca2+/calmodulin (CaM)-dependent protein kinase kinases (CaMKK2) contribute to the ghrelin activation of NPY neurons.

Download (371.02 kB)
figure
posted on 2017-09-06, 17:41 authored by Hiroshi Hashiguchi, Zhenyu Sheng, Vanessa Routh, Volodymyr Gerzanich, J. Marc Simard, Joseph Bryan

(A) AICAR (400 μM), an AMPK activator, mimics the effect of ghrelin. (B) Compound-C (30 μM), an inhibitor of AMPK, completely blocks the effect of oligomycin. (C) STO-609 (1 μM), a CAMKK2 inhibitor, partially reverses the effect of ghrelin. Ghrelin was applied at 100 pM (red bars). Traces are mean values from 5–20 cells.

History

Usage metrics

Read the peer-reviewed publication

Direct versus indirect actions of ghrelin on hypothalamic NPY neurons
PLOS ONEPLOS ONE

Categories

Keywords

CNQXTTA-PNPY-GFP neuronsSUR 1 agonistNPY GFP-labeled neuronsTrpm 4 antagonistsTrpm 4 modulatorsTTXpresynaptic action potentialsPKAKir 6.2ECAMPKghrelin actionMPNPY neuronsSUR 1growth hormone secretagogue receptorSUR 1 antagonistConclusions NPY neuronsNPY neurons ObjectivesPLCSNX 482-sensitive R-type voltagemeasure ion channel activityIRResults Acylated ghrelin depolarizedGHS-RGABA

Licence

CC BY 4.0

Exports