AHR signaling contributes to HTLV-1 plus-strand expression.
(A, B, E) HPB-ATL-T, MT-2, MT-4 and TL-Om1 cells were infected with lentivirus against AHR. The screened AHR-knockdown cells were subjected to analyze the expression by (A, E) quantitative RT-PCR and (B, E) immunoblot. (C, D, F) HPB-ATL-T, MT-2, MT-4 and TL-Om1 cells were treated with CH-223191 (10, 20 μM) or DMSO for 24 h, then subjected to analyze the expression by (C, F) quantitative RT-PCR and (D) immunoblot. Results of quantitative RT-PCR are shown as relative mRNA expression normalized to that of 18S rRNA. shNC, negative control of shRNA. *, p<0.05. **, p<0.01. NS, not significant.